Journal: Biomolecules

Article Title: UT-1 Transporter Expression in the Spiny Dogfish (Squalus acanthias): UT-1 Protein Shows a Different Localization in Comparison to That of Other Sharks

doi: 10.3390/biom14091151

Figure Lengend Snippet: Figure 4. Western blots of Squalus acanthias protein crude extracts (400 µg; (A,C)) or purified plasma membranes (300 µg; (B)) from kidney and or brain tissue as indicated. Molecular Weight Protein marker (M) was Precision Plus Protein Kaleidoscope marker (Biorad, Santa Rosa, CA, USA). Peptide block refers to blots incubated with antibody pre-blocked (50 µg peptide/mL for >1 h) with the peptide antigen used to make the antibody. For the UT-1 antibody blot, the blot was one of four blot strips from one filter, with only one marker lane (M) which was located on a different strip, and so that was scanned separately and aligned with the UT-1 blot. All the peptide-blocked blot lanes were also cropped and slightly resized to align them next to each blot to aid comparison. Original scans are available on request.

Article Snippet: Molecular Weight Protein marker (M) was Precision Plus Protein Kaleidoscope marker (Biorad, Santa Rosa, CA, USA).

Techniques: Western Blot, Purification, Clinical Proteomics, Molecular Weight, Marker, Blocking Assay, Incubation, Stripping Membranes, Comparison